Journal: International Journal of Exercise Science

Article Title: Effects of Varying Load Intensity on Skeletal Muscle Damage Between Two Isovolumic Resistance Exercise Bouts

doi:

Figure Lengend Snippet: a. Plasma myoglobin protein expression for baseline, LHL, and HHL. b. Plasma CRP protein expression for Base, LHL, and HHL. * indicates significant difference from Base (p< 0.05). Bar graph data represents mean value ± SEM.

Article Snippet: Once all eleven participants completed the study, plasma samples were thawed at room temperature and analyzed via enzyme linked immunosorbent assay for potential differences in plasma myoglobin (Abcam; Cambridge, MA; ab171580) and CRP (Cayman Chemicals; Ann Arbor, MI; #10011236).

Techniques: Expressing

Journal: BioResearch Open Access

Article Title: Allogeneic Transplantation of Periodontal Ligament-Derived Multipotent Mesenchymal Stromal Cell Sheets in Canine Critical-Size Supra-Alveolar Periodontal Defect Model

doi: 10.1089/biores.2015.0043

Figure Lengend Snippet: Evaluation of immunological reaction in response to allogeneic and autologous transplantation of periodontal ligament cell sheets by enzyme-linked immunosorbent assay. (A) Concentrations of serum C-reactive protein (mg/dL). (B) Concentrations of serum cluster of differentiation 30 (pg/mL). (C) Concentrations of serum interferon-γ (pg/mL). (D) Concentrations of serum interleukin-10 (pg/mL). (A–D) There were no significant differences in the concentrations of these inflammatory markers between the allogeneic and autologous groups at any time point. Open and closed circles indicate actual values for the allogeneic and autologous groups, respectively. Open and closed bars indicate averaged values for the allogeneic and autologous groups, respectively.

Article Snippet: Serum C-reactive protein (CRP), serum interleukin-10 (IL-10), serum interferon-γ (IFN-γ), and serum cluster of differentiation 30 (CD30) were determined using commercially available canine-specific enzyme-linked immunosorbent assay (ELISA) kits: CRP canine high-sensitivity ELISA Kit (Helica Biosystems, Santa Ana, CA); Quantikine canine IL-10; canine IFN-γ (R&D Systems); and canine CD30 ELISA Kit (Novateinbiosciences, Cambridge, MA) ( n = 7 or 8 dogs).

Techniques: Transplantation Assay, Enzyme-linked Immunosorbent Assay

Journal:

Article Title: Monocyte-specific Bcl-2 expression attenuates inflammation and heart failure in monocyte chemoattractant protein-1 (MCP-1)-induced cardiomyopathy ⋆

doi: 10.1016/j.cardiores.2006.03.008

Figure Lengend Snippet: Bcl-2 expression in monocytes results in reduced inflammatory cells infiltration in the hearts of MCP mice. (A) Representative photomicrographs demonstrating immunohistochemical staining with anti-CD45, -Mac-1 and -Mac-3 antibodies in heart sections from 6-month-old wild-type and transgenic mice. Positive-stained cells were visualized with diaminobenzidine (brown). (B–D) Histograms showing the number of CD45-, Mac-1- and Mac-3-positive cells in the hearts of wild-type, MCP and MCP/Bcl-2 mice. *P <0.001 versus wild-type mice; #P <0.05 versus wild-type and MCP mice; n =6 per group per time point. (E) Circulating levels of CRP assayed by ELISA. n =6 per group per time point. *P <0.05 versus wild-type and MCP/Bcl-2 mice; #P <0.05 versus wild-type mice.

Article Snippet: The circulating levels of circulating C-reactive protein (CRP) in plasma were measured with mouse high-sensitive CRP ELISA kit (Kamiya Biomedical Company, Seattle, WA), which specifically detects mouse CRP.

Techniques: Expressing, Immunohistochemical staining, Staining, Transgenic Assay, Enzyme-linked Immunosorbent Assay